The purpose of this study was to develop “The Test of Concepts in Ecology and Environment for Secondary School Students” and to investigate the junior high students’ conceptions of ecology and environment. This test was constructed based on related literature review, content analysis of textbooks, and interviews with high school students. This test and “The Test of Concepts of Bioenergetics” previously developed by the author were used in the study. 1735 students sampled from 50 classes in 16 junior high schools located in Chia-Nan area participated in this study. They all took the two aforementioned tests. Totally, 1702 questionnaires were complete. The data collected were computed basic statistics and analyzed with ANOVA and multiple regression to predict effects of environmental education by bioenergetics understanding, gender difference, and other backgrounds. Major results were as follows: first, there were significant differences in conceptions about ecological environment among the students with different genders, parents’ educational backgrounds and occupations, living environments, and school locations; second, the effects of environmental education could be predicted by bioenergetics understandings, gender, and other backgrounds; and third, .the students still had many alternative conceptions in ecological environment concepts after learning.
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